Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Antimicrobial Activity of Human Fetal Membranes: From Biological Function to Clinical Use

doi: 10.3389/fbioe.2021.691522

Figure Lengend Snippet: Protocols for preparation of hACM, hAM, and hCM and their derivatives, which were used for investigation of antimicrobial activity. There are many variations in protocols for the preparation of fetal membranes to be used as an antimicrobial agent, which underlines the need for protocol standardization before considering clinical use.

Article Snippet: hCVAM conditioned medium , Human term placental tissues were obtained from commercial tissue agencies and processed and Osiris Therapeutics, Inc. (Columbia, MD, United States) following the proprietary manufacturing procedure. Namely, the hAM separated from the placenta within 36 h of the collection and incubated in the DMEM culture medium containing gentamicin, vancomycin, and amphotericin B for 18-48 h at 37°C and 5% CO 2 . Residual antibiotics were removed by washing the hAM with Dulbecco’s PBS and hAM was cut into pieces. Cryopreservation was performed by freezing hAM in DMSO containing cryoprotectant at a controlled cooling rate, according to the proprietary process developed by Osiris Therapeutics, Inc. Before use, hCVAM was thawed at room temperature and rinsed in PBS. To prepare conditioned medium from hCVAM, the hCVAM was incubated for 4/20/24 h in DMEM, supplemented with 10% FBS (1 ml of culture medium per 4 cm 2 of hCVAM) on a shaker. , Yes , .

Techniques: Activity Assay, Sterility, Saline, Incubation, In Vitro, Pore Size

Journal: Advances in Wound Care

Article Title: Use of Placental Membranes for the Treatment of Chronic Diabetic Foot Ulcers

doi: 10.1089/wound.2015.0634

Figure Lengend Snippet: Classification of current commercial placental membrane products (excluding amniotic fluid-based products)

Article Snippet: Decellularized Placental Tissue , Dehydrated , Biovance , Amnion , Alliqua (Celgene) , http://alliqua.com/products/biovance/.

Techniques: Membrane, Preserving

Journal: Advances in Wound Care

Article Title: Use of Placental Membranes for the Treatment of Chronic Diabetic Foot Ulcers

doi: 10.1089/wound.2015.0634

Figure Lengend Snippet: Classification of current commercial placental membrane products (excluding amniotic fluid-based products)

Article Snippet: Devitalized placental tissue , Cryopreserved , Neox , Amnion , Amniox Medical , www.amnioxmedical.com/.

Techniques: Membrane, Preserving